16.12.2021

Research assissant (PhD student) (m/f/d)

Leibniz Research Centre for Working Environment and Human Factors (IfADo), Dortmund

‘Forschungsgesellschaft für Arbeitsphysiologie und Arbeitsschutz e.V. Dortmund’ (legal entity name) is a research institution institutionally funded by the German Federal Ministry of Labor and Social Affairs and the State of North Rhine-Westphalia. It is the sponsor of ’Leibniz Research Centre for Working Environment and Human Factors at TU Dortmund’ (IfADo). IfADo explores the risks and potentials of modern work in interdisciplinary approaches. We combine the disciplines of Ergonomics, Immunology, Toxicology, Psychology and Neurosciences.

The Department of Toxicology is looking for the following to start as soon as possible

a research assissant (PhD student)(m/f/d)

The part-time position (65%) is available immediately and is initially limited to 3 years.

Project description:

The rewiring of a cell’s metabolic capacity is now an accepted feature of cancer. However, the exact mechanism underlying these changes is still not fully understood. Lysophosphatidic acid (LPA) is a bioactive lipid that serves as an important intermediate in the synthesis and breakdown of phospholipids. It also acts as a ligand for G protein-coupled receptors at the cell membrane, leading to the activation of signaling pathways that regulate processes altered in cancer, such as survival, proliferation and migration. There are also reports that LPA levels are elevated in ovarian cancer patients. The majority of the work on LPA in cancer has focused on LPA in the extracellular environment. However, recent work by us have shown that LPA inside the cell is also associated with migration of ovarian cancer cells, as well as tumor growth of an ovarian carcinoma xenograft model, but the mechanism remains unknown. Therefore, to better understand the role of intracellular LPA in ovarian cancer, a characterization of the metabolic pathways regulating LPA levels in tumor cell lines is planned using molecular biology approaches in high grade ovarian cancer cell lines, and the effect of gene manipulation on a wide array of phenotypic endpoints, as well as intracellular lipid and metabolite levels. Finally, the potential clinical relevance of findings in vitro will be investigated using animal models of ovarian cancer.

The project, which is funded by the DFG, will be conducted at the Leibniz-Institute for Working Environment and Human Factors at TU Dortmund (IfADo), which offers excellent research conditions, including well-equipped S1 and S2 cell and molecular biology labs, an animal facility, an imaging facility with confocal, two-photon, and RAMAN microscopes, a high throughput microscopy platform, microCT, MRI and the IVIS Spectrum for whole animal imaging, a histology laboratory, analytical chemistry facility for proteomics and metabolomics analyses, FACS facility and a sequencing platform.

Your Profile:

  • Completed master’s degree or diploma in the field of molecular biology, biochemistry, biotechnology, biology, pharmacy or a related course of studies
  • Experience with working with animals (FELASA B or C) or willing to learn
  • Experience in cloning, cell culture, qRT-PCR, western blotting, immunohistochemistry and immunocytochemistry would additionally be beneficial
  • Ability to work independently as well as part of a team
  • Good multitasking, time management and data organization skills
  • English proficiency in speaking and writing

We offer:

  • A part-time position with currently 25,89 working hours per week
  • Remuneration according to the collective agreement for the public service (position EG 13 TV-L. The classification depends on the collective agreement and personal requirements) 
  • All social benefits provided by working for the public service: e.g. an annual bonus, payment of benefits
  • Flexible working time models for the compatibility of family and career
  • An interesting, professional challenge in which you can contribute and expand your expertise and competence with a high degree of independence in an interdisciplinary team
  • An exciting task and many opportunities for further training

The IfADo promotes professional equality for all genders and is certified accordingly.

We value diversity and therefore welcome all applications - regardless of gender, disability, nationality or ethnic and social origin.

If you have any questions, please contact Dr. Rosemarie Marchan marchan(at)ifado.de.

Have we aroused your interest?

Then please send your meaningful application documents with all relevant certificates including the reference code "DFG Marchan" until 31th of January 2022, preferably by mail to bewerbung(at)ifado.de.

Alternative you can send your application* by post to the following address: Forschungsgesellschaft für Arbeitsphysiologie und Arbeitsschutz e.V., Personalabteilung, Ardeystraße 67, 44139 Dortmund.

*(We ask that application documents be submitted preferably by email or only in copies and not in folders, as the documents will not be returned. These will be destroyed in accordance with data protection regulations after the retention period has expired.)

General information about the Research Centre can be found here www.ifado.de.